Package: NORMAgene 0.1.1
Grant C. OConnell
NORMAgene: Data-Driven qRT-PCR Normalization Using NORMAgene
Enables correction for technical variance in raw quantitative reverse transcription polymerase chain reaction (qRT-PCR) data using the least squares-based NORMAgene data-driven normalization algorithm originally described by Heckmann et al. (2011) <doi:10.1186/1471-2105-12-250>. Performs normalization of raw crossing threshold values (CT) and also calculates relative variability metrics that can be used to assess the impact of normalization on variance.
Authors:
NORMAgene_0.1.1.tar.gz
NORMAgene_0.1.1.tar.gz(r-4.7-any)NORMAgene_0.1.1.tar.gz(r-4.6-any)
NORMAgene_0.1.1.tgz(r-4.6-emscripten)
manual.pdf |manual.html✨
DESCRIPTION
card.svg |card.png
NORMAgene/json (API)
| # Install 'NORMAgene' in R: |
| install.packages('NORMAgene', repos = c('https://cran.r-universe.dev', 'https://cloud.r-project.org')) |
- multi_cond_data - Example dataset from a multi-condition qPCR experiment.
- single_cond_data - Example dataset from a single condition qPCR experiment.
This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.
Last updated from:ae1443615b. Checks:4 OK. Indexed: yes.
| Target | Result | Time | Files | Syslog |
|---|---|---|---|---|
| linux-devel-x86_64 | OK | 107 | ||
| source / vignettes | OK | 151 | ||
| linux-release-x86_64 | OK | 115 | ||
| wasm-release | OK | 87 |
Exports:correction_factorsnorma_generelative_variability
Dependencies:
Readme and manuals
Help Manual
| Help page | Topics |
|---|---|
| Data-Driven qRT-PCR Normalization Using NORMAgene | NORMAgene-package |
| Retrieve scaling factors from NORMAgene output | correction_factors |
| Example dataset from a multi-condition qPCR experiment. | multi_cond_data |
| Normalize CT values using NORMAgene | norma_gene |
| Retrieve relative variability metrics from NORMAgene output | relative_variability |
| Example dataset from a single condition qPCR experiment. | single_cond_data |