---
title: "qPCRhelper"
output: rmarkdown::html_vignette
vignette: >
  %\VignetteIndexEntry{qPCRhelper}
  %\VignetteEngine{knitr::rmarkdown}
  %\VignetteEncoding{UTF-8}
---

```{r, include = FALSE}
knitr::opts_chunk$set(
  collapse = TRUE,
  comment = "#>"
)
```

```{r setup}
library(qPCRhelper)
library(ggplot2)
#Generate sample data
Sample <- c("C1", "C2", "T1", "T2")
Group <- c("Control", "Control", "Treatment", "Treatment")
GAPDH <- c("18.1", "18.2", "17.9", "18.0")
IL4R <- c("32.4", "32.8", "20.2", "21.3")
qpcr_data <- data.frame(Sample, Group, GAPDH, IL4R)
write.table(qpcr_data, file = "qpcr_data.txt", sep = "\t", row.names = F)

qpcr_out <- qPCRhelper(data.dir = "./qpcr_data.txt", ref.gene = "GAPDH", ref.group = "Control")
```