{
  "_id": "6ac67aefca1b382b34ef3e44",
  "Package": "easybio",
  "Title": "Comprehensive Single-Cell Annotation and Transcriptomic Analysis\nToolkit",
  "Version": "1.3.0",
  "Authors@R": "person(\"Wei\", \"Cui\", email = \"m2c.w@outlook.com\", role = c(\"aut\", \"cre\", \"cph\"), comment = c(ORCID = \"0009-0004-8315-5899\"))",
  "Description": "Provides a comprehensive toolkit for single-cell\nannotation with the 'CellMarker 3.0' database\n<https://bio-bigdata.hrbmu.edu.cn/CellMarker/>. Streamlines\nbiological label assignment in single-cell RNA-seq data and\nfacilitates transcriptomic analysis, including preparation of\nTCGA<https://portal.gdc.cancer.gov/> and\nGEO<https://www.ncbi.nlm.nih.gov/geo/> datasets, differential\nexpression analysis and visualization of enrichment analysis\nresults. Additional utility functions support various\nbioinformatics workflows. See Wei Cui (2024) <doi:\n10.1101/2024.09.14.609619> for more details.",
  "URL": "https://github.com/person-c/easybio",
  "BugReports": "https://github.com/person-c/easybio/issues",
  "License": "MIT + file LICENSE",
  "Encoding": "UTF-8",
  "RoxygenNote": "7.3.2",
  "LazyData": "true",
  "biocViews": "limma, GEOquery, edgeR, fgsea",
  "Language": "en-US",
  "VignetteBuilder": "litedown",
  "Config/testthat/edition": "3",
  "NeedsCompilation": "no",
  "Packaged": {
    "Date": "2026-10-07 16:52:16 UTC",
    "User": "root"
  },
  "Author": "Wei Cui [aut, cre, cph] (ORCID:\n<https://orcid.org/0009-0004-8315-5899>)",
  "Maintainer": "Wei Cui <m2c.w@outlook.com>",
  "Repository": "https://cran.r-universe.dev",
  "Date/Publication": "2026-10-07 16:41:31 UTC",
  "RemoteUrl": "https://github.com/cran/easybio",
  "RemoteRef": "HEAD",
  "RemoteSha": "6010abbdbc7ed3a83683413997d24a3794ecda9b",
  "_user": "cran",
  "_type": "src",
  "_file": "easybio_1.3.0.tar.gz",
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  "_expires": "2027-01-15T17:01:32.000Z",
  "_created": "2026-10-07T16:52:16.000Z",
  "_published": "2026-10-07T17:01:35.355Z",
  "_buildurl": "https://github.com/r-universe/cran/actions/runs/37654840319",
  "_status": "success",
  "_host": "GitHub-Actions",
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  "_upstream": "https://github.com/cran/easybio",
  "_commit": {
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    "author": "Wei Cui <m2c.w@outlook.com>",
    "committer": "cran-robot <csardi.gabor+cran@gmail.com>",
    "message": "version 1.3.0\n",
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  "_maintainer": {
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    "email": "m2c.w@outlook.com",
    "login": "person-c",
    "orcid": "0009-0004-8315-5899",
    "description": "",
    "uuid": 93318795
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  "_distro": "resolute",
  "_registered": true,
  "_dependencies": [
    {
      "package": "R",
      "version": ">= 4.1.0",
      "role": "Depends"
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    {
      "package": "data.table",
      "version": ">= 1.15.0",
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    },
    {
      "package": "checkmate",
      "role": "Imports"
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    {
      "package": "ggplot2",
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    },
    {
      "package": "httr2",
      "role": "Imports"
    },
    {
      "package": "lifecycle",
      "role": "Imports"
    },
    {
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    },
    {
      "package": "xml2",
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    },
    {
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    },
    {
      "package": "patchwork",
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    },
    {
      "package": "ggrepel",
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    },
    {
      "package": "Seurat",
      "role": "Suggests"
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    {
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    },
    {
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      "role": "Suggests"
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    {
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      "role": "Suggests"
    },
    {
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      "role": "Suggests"
    },
    {
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  "_owner": "cran",
  "_selfowned": false,
  "_usedby": 0,
  "_updates": [
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  ],
  "_tags": [
    {
      "name": "1.3.0",
      "date": "2026-10-07"
    }
  ],
  "_topics": [
    "limma",
    "geoquery",
    "edger",
    "fgsea"
  ],
  "_stars": 1,
  "_contributors": [
    {
      "user": "person-c",
      "count": 8,
      "uuid": 93318795
    }
  ],
  "_userbio": {
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    "type": "organization",
    "name": "cran",
    "followers": 630,
    "description": "Unofficial read-only mirror of all CRAN R packages"
  },
  "_downloads": {
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    "source": "https://cranlogs.r-pkg.org/downloads/total/last-month/easybio"
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  "_devurl": "https://github.com/person-c/easybio",
  "_searchresults": 48,
  "_rbuild": "4.6.1",
  "_assets": [
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    "extra/citation.html",
    "extra/citation.json",
    "extra/citation.txt",
    "extra/contents.json",
    "extra/easybio.html",
    "extra/manual.pdf",
    "extra/NEWS.html",
    "extra/NEWS.txt",
    "extra/readme.html",
    "extra/readme.md",
    "LICENSE"
  ],
  "_homeurl": "https://github.com/person-c/easybio",
  "_realowner": "person-c",
  "_cranurl": false,
  "_releases": [
    {
      "version": "1.0",
      "date": "2024-09-05"
    },
    {
      "version": "1.0.1",
      "date": "2024-09-08"
    },
    {
      "version": "1.1.0",
      "date": "2024-09-16"
    },
    {
      "version": "1.1.1",
      "date": "2025-02-12"
    },
    {
      "version": "1.2.1",
      "date": "2025-08-30"
    },
    {
      "version": "1.2.2",
      "date": "2025-09-01"
    },
    {
      "version": "1.2.3",
      "date": "2025-10-03"
    }
  ],
  "_exports": [
    "Artist",
    "available_ele",
    "available_tissue_class",
    "available_tissue_type",
    "check_marker",
    "dge_list",
    "dgeList",
    "dprocess_dgeList",
    "finsert",
    "get_attr",
    "get_marker",
    "group_stat",
    "group_stat_i",
    "groupStat",
    "groupStatI",
    "limma_fit",
    "limmaFit",
    "list2dt",
    "list2graph",
    "match_ref",
    "matchCellMarker2",
    "plot_enrichment",
    "plot_gsea",
    "plot_marker_distribution",
    "plot_ora",
    "plot_possible_cell",
    "plot_rank",
    "plot_seurat_dot",
    "plot_volcano",
    "plotEnrichment2",
    "plotGSEA",
    "plotMarkerDistribution",
    "plotORA",
    "plotPossibleCell",
    "plotRank",
    "plotSeuratDot",
    "plotVolcano",
    "prepare_geo",
    "prepare_tcga",
    "process_dge_list",
    "set_colnames",
    "set_rownames",
    "set_savedir",
    "setcolnames",
    "setrownames",
    "setSavedir",
    "split_matrix",
    "suggest_best_match",
    "theme_publication",
    "tune_parameters",
    "tuneParameters",
    "uniprot_id_map",
    "work_in",
    "workIn"
  ],
  "_datasets": [
    {
      "name": "CHOL_DEGs",
      "title": "Example DEGs data from Limma-Voom workflow for TCGA-CHOL project",
      "object": "CHOL_DEGs",
      "class": [
        "data.table",
        "data.frame"
      ],
      "fields": [
        "rid",
        "seqnames",
        "start",
        "end",
        "width",
        "strand",
        "source",
        "type",
        "score",
        "phase",
        "gene_id",
        "gene_type",
        "gene_name",
        "level",
        "hgnc_id",
        "havana_gene",
        "logFC",
        "AveExpr",
        "t",
        "P.Value",
        "adj.P.Val",
        "B",
        "tumor_vs_normal"
      ],
      "rows": 21938,
      "table": true,
      "tojson": true
    },
    {
      "name": "pbmc.markers",
      "title": "Example marker data from Seurat::FindAllMarkers()",
      "object": "pbmc.markers",
      "class": [
        "data.frame"
      ],
      "fields": [
        "p_val",
        "avg_log2FC",
        "pct.1",
        "pct.2",
        "p_val_adj",
        "cluster",
        "gene"
      ],
      "rows": 11629,
      "table": true,
      "tojson": true
    }
  ],
  "_help": [
    {
      "page": "Artist",
      "title": "Visualization Artist for Custom Plots",
      "topics": [
        "Artist"
      ]
    },
    {
      "page": "available_ele",
      "title": "Extract Unique Elements from a Column with Optional Filtering",
      "topics": [
        "available_ele"
      ]
    },
    {
      "page": "available_tissue_class",
      "title": "Retrieve Available Tissue Classes for a Given Species",
      "topics": [
        "available_tissue_class"
      ]
    },
    {
      "page": "available_tissue_type",
      "title": "Retrieve Available Tissue Types for a Given Species",
      "topics": [
        "available_tissue_type"
      ]
    },
    {
      "page": "check_marker",
      "title": "Verify and Explore Cell Type Annotations",
      "topics": [
        "check_marker"
      ]
    },
    {
      "page": "CHOL_DEGs",
      "title": "Example DEGs data from Limma-Voom workflow for TCGA-CHOL project",
      "topics": [
        "CHOL_DEGs"
      ]
    },
    {
      "page": "dge_list",
      "title": "Construct a DGEList Object",
      "topics": [
        "dge_list"
      ]
    },
    {
      "page": "dgeList",
      "title": "Construct a DGEList Object (Deprecated)",
      "topics": [
        "dgeList"
      ]
    },
    {
      "page": "dprocess_dgeList",
      "title": "Filter and Normalize DGEList Data (Deprecated)",
      "topics": [
        "dprocess_dgeList"
      ]
    },
    {
      "page": "finsert",
      "title": "Create a Vector from an Index-to-Label Map",
      "topics": [
        "finsert"
      ]
    },
    {
      "page": "get_attr",
      "title": "Retrieve Attributes from an R Object",
      "topics": [
        "get_attr"
      ]
    },
    {
      "page": "get_marker",
      "title": "Retrieve Markers for Specific Cells from cellMarker3",
      "topics": [
        "get_marker"
      ]
    },
    {
      "page": "group_stat",
      "title": "Perform Summary Analysis by Group Using Regular Expressions",
      "topics": [
        "group_stat"
      ]
    },
    {
      "page": "group_stat_i",
      "title": "Perform Summary Analysis by Group Using an column Index",
      "topics": [
        "group_stat_i"
      ]
    },
    {
      "page": "groupStat",
      "title": "Summarize Data by Group Using Regular Expressions (Deprecated)",
      "topics": [
        "groupStat"
      ]
    },
    {
      "page": "groupStatI",
      "title": "Summarize Data by Group Using an Index (Deprecated)",
      "topics": [
        "groupStatI"
      ]
    },
    {
      "page": "limma_fit",
      "title": "Fit a Linear Model for RNA-seq data using limma",
      "topics": [
        "limma_fit"
      ]
    },
    {
      "page": "limmaFit",
      "title": "Fit a Linear Model for RNA-seq Data (Deprecated)",
      "topics": [
        "limmaFit"
      ]
    },
    {
      "page": "list2dt",
      "title": "Convert a List with Vector Values to a Long Data.table",
      "topics": [
        "list2dt"
      ]
    },
    {
      "page": "list2graph",
      "title": "Convert a Named List into a Graph Based on Overlap",
      "topics": [
        "list2graph"
      ]
    },
    {
      "page": "match_ref",
      "title": "Annotate Clusters by Matching Markers with the CellMarker 3.0 Database",
      "topics": [
        "match_ref"
      ]
    },
    {
      "page": "matchCellMarker2",
      "title": "Annotate Clusters by Matching Markers (Deprecated)",
      "topics": [
        "matchCellMarker2"
      ]
    },
    {
      "page": "pbmc.markers",
      "title": "Example marker data from Seurat::FindAllMarkers()",
      "topics": [
        "pbmc.markers"
      ]
    },
    {
      "page": "plot_enrichment",
      "title": "Plot Enrichment for a Specific Pathway in fgsea",
      "topics": [
        "plot_enrichment"
      ]
    },
    {
      "page": "plot_gsea",
      "title": "Visualization of GSEA Result from 'fgsea::fgsea()'",
      "topics": [
        "plot_gsea"
      ]
    },
    {
      "page": "plot_marker_distribution",
      "title": "Plot Distribution of a Marker Across Tissues and Cell Types",
      "topics": [
        "plot_marker_distribution"
      ]
    },
    {
      "page": "plot_ora",
      "title": "Visualization of ORA Test Results",
      "topics": [
        "plot_ora"
      ]
    },
    {
      "page": "plot_possible_cell",
      "title": "Plot Possible Cell Distribution Based on match_ref() Results",
      "topics": [
        "plot_possible_cell"
      ]
    },
    {
      "page": "plot_rank",
      "title": "Visualization of GSEA Rank Statistics",
      "topics": [
        "plot_rank"
      ]
    },
    {
      "page": "plot_seurat_dot",
      "title": "Create a Dot Plot to Visualize Marker Gene Expression",
      "topics": [
        "plot_seurat_dot"
      ]
    },
    {
      "page": "plot_volcano",
      "title": "Plot Volcano Plot for Differentially Expressed Genes",
      "topics": [
        "plot_volcano"
      ]
    },
    {
      "page": "plotEnrichment2",
      "title": "Plot Enrichment for a Pathway (Deprecated)",
      "topics": [
        "plotEnrichment2"
      ]
    },
    {
      "page": "plotGSEA",
      "title": "Visualize GSEA Results (Deprecated)",
      "topics": [
        "plotGSEA"
      ]
    },
    {
      "page": "plotMarkerDistribution",
      "title": "Plot Distribution of a Marker (Deprecated)",
      "topics": [
        "plotMarkerDistribution"
      ]
    },
    {
      "page": "plotORA",
      "title": "Visualize ORA Test Results (Deprecated)",
      "topics": [
        "plotORA"
      ]
    },
    {
      "page": "plotPossibleCell",
      "title": "Plot Possible Cell Distribution (Deprecated)",
      "topics": [
        "plotPossibleCell"
      ]
    },
    {
      "page": "plotRank",
      "title": "Visualize GSEA Rank Statistics (Deprecated)",
      "topics": [
        "plotRank"
      ]
    },
    {
      "page": "plotSeuratDot",
      "title": "Create a Dot Plot of Marker Gene Expression (Deprecated)",
      "topics": [
        "plotSeuratDot"
      ]
    },
    {
      "page": "plotVolcano",
      "title": "Plot a Volcano Plot (Deprecated)",
      "topics": [
        "plotVolcano"
      ]
    },
    {
      "page": "prepare_geo",
      "title": "Download and Process GEO Data",
      "topics": [
        "prepare_geo"
      ]
    },
    {
      "page": "prepare_tcga",
      "title": "Prepare TCGA Data for Analysis",
      "topics": [
        "prepare_tcga"
      ]
    },
    {
      "page": "process_dge_list",
      "title": "Filter Low-Expressed Genes and Normalize DGEList Data",
      "topics": [
        "process_dge_list"
      ]
    },
    {
      "page": "set_colnames",
      "title": "Rename Column Names of a Data Frame or Matrix",
      "topics": [
        "set_colnames"
      ]
    },
    {
      "page": "set_rownames",
      "title": "Rename Row Names of a Data Frame or Matrix",
      "topics": [
        "set_rownames"
      ]
    },
    {
      "page": "set_savedir",
      "title": "Set a Directory for Saving Files",
      "topics": [
        "set_savedir"
      ]
    },
    {
      "page": "setcolnames",
      "title": "Rename Column Names (Deprecated)",
      "topics": [
        "setcolnames"
      ]
    },
    {
      "page": "setrownames",
      "title": "Rename Row Names (Deprecated)",
      "topics": [
        "setrownames"
      ]
    },
    {
      "page": "setSavedir",
      "title": "Set a Directory for Saving Files (Deprecated)",
      "topics": [
        "setSavedir"
      ]
    },
    {
      "page": "split_matrix",
      "title": "Split a Matrix into Smaller Sub-matrices by Column or Row",
      "topics": [
        "split_matrix"
      ]
    },
    {
      "page": "suggest_best_match",
      "title": "Suggest Best Matches for a String from a Vector of Choices",
      "topics": [
        "suggest_best_match"
      ]
    },
    {
      "page": "theme_publication",
      "title": "Custom ggplot2 Theme for Academic Publications",
      "topics": [
        "theme_publication"
      ]
    },
    {
      "page": "tune_parameters",
      "title": "Optimize Resolution and Gene Number Parameters for Cell Type Annotation",
      "topics": [
        "tune_parameters"
      ]
    },
    {
      "page": "tuneParameters",
      "title": "Tune Parameters for Cell Type Annotation (Deprecated)",
      "topics": [
        "tuneParameters"
      ]
    },
    {
      "page": "uniprot_id_map",
      "title": "Map UniProt IDs to Other Identifiers",
      "topics": [
        "uniprot_id_map"
      ]
    },
    {
      "page": "work_in",
      "title": "Perform Operations in a Specified Directory and Return to the Original Directory",
      "topics": [
        "work_in"
      ]
    },
    {
      "page": "workIn",
      "title": "Perform Operations in a Directory (Deprecated)",
      "topics": [
        "workIn"
      ]
    }
  ],
  "_readme": "https://github.com/cran/easybio/raw/HEAD/README.md",
  "_rundeps": [
    "askpass",
    "backports",
    "checkmate",
    "cli",
    "cpp11",
    "curl",
    "data.table",
    "farver",
    "ggplot2",
    "glue",
    "gtable",
    "httr2",
    "isoband",
    "labeling",
    "lifecycle",
    "magrittr",
    "openssl",
    "R6",
    "RColorBrewer",
    "rlang",
    "S7",
    "scales",
    "sys",
    "vctrs",
    "viridisLite",
    "withr",
    "xml2"
  ],
  "_vignettes": [
    {
      "source": "example_limma.Rmd",
      "filename": "example_limma.html",
      "title": "Example Workflow for Bulk RNA-seq Analysis with limma-voom",
      "author": "cw",
      "engine": "litedown::vignette",
      "headings": [
        "Limma-voom workflow",
        "Prepare data",
        "Example Workflow: TCGA CHOL Project"
      ],
      "created": "2025-08-30 06:40:03",
      "modified": "2026-10-07 16:41:31",
      "commits": 2
    },
    {
      "source": "example-sc-seq-workflow.Rmd",
      "filename": "example-sc-seq-workflow.html",
      "title": "Example Workflow for Single-Cell Annotation with easybio",
      "author": "cw",
      "engine": "litedown::vignette",
      "headings": [
        "Introduction",
        "Setup",
        "Step 1: Automated Annotation with match_ref",
        "Bounding the search to the right tissue",
        "Step 2: Verification and Exploration",
        "Answering \"Why was this annotation made?\"",
        "Answering \"Is this annotation correct?\"",
        "Visual Confirmation with plot_seurat_dot",
        "Step 3: Final Manual Curation",
        "Using a Custom Marker Database",
        "Additional Utilities",
        "get_marker()",
        "plot_marker_distribution()"
      ],
      "created": "2025-08-30 06:40:03",
      "modified": "2026-10-07 16:41:31",
      "commits": 3
    },
    {
      "source": "example-artist.Rmd",
      "filename": "example-artist.html",
      "title": "Exploratory Data Visualization with the Artist Class",
      "author": "cw",
      "engine": "litedown::vignette",
      "headings": [
        "Introduction",
        "Setup",
        "Basic Usage",
        "Statistical Tests and Auto-Annotation",
        "Method Chaining",
        "Data Transformation with fun",
        "Available Plot Types",
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