--- title: "SMAHP" author: "Weijia Fu, Seungjun Ahn" date: "Feb 5th, 2025" output: rmarkdown::html_vignette vignette: > %\VignetteIndexEntry{SMAHP} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r, include = FALSE} knitr::opts_chunk$set( collapse = TRUE, warning = FALSE, comment = "#>" ) ``` ## Introduction Welcome to the SMAHP vignette! This document provides an overview of the SMAHP package. SMAHP (Survival Mediation Analysis of High-dimensional Proteogenomic data) is a novel method for survival mediation analysis that simultaneously handles high-dimensional exposures and mediators, integrates multi-omics data, and offers a robust statistical framework for identifying causal pathways on survival outcomes. SMAHP package is a software extension of our methodology work. ### Installation To install the package, please use: ```{r} #install.packages("SMAHP") ``` ## Example Usage We will use the example data built in the package to demonstrate how to use the main function. ```{r} library(SMAHP) # load example data data("example_dat") ``` The example data is a simulated dataset for demonstration purpose. It contains exposure (X), mediators (M), covariates (C) and survival data (surv_dat). ```{r} surv_dat <- example_dat$surv_dat res <- SMAHP(example_dat$X, example_dat$M, example_dat$C, time = surv_dat$time, status = surv_dat$status) ``` ```{r} print(res$p_final_matrix) ``` ```{r} print(res$p_adjusted_matrix) ``` ```{r} print(res$p_med_matrix) ``` The main function returns a list containing three matrices: (1) p_final_matrix, which is the raw p-value before adjustment, (2) p_adjusted_matrix, which contains p-values adjusted using the selected adjustment method, and (3) p_med_matrix, a mediation-exposure matrix encoded with 0s and 1s. In p_med_matrix, a value of 1 indicates a detected mediation association, while 0 means no significant mediation association. The NAs in p_final_matrix and p_adjusted_matrix mean the exposure-mediator pairs were not selected in previous steps. The function get_sig_pathway can be used to extract the the name of exposure and mediator from identified significant mediation pathways with related adjusted p-values. ```{r} get_sig_pathway(res_SMAHP = res) ``` The function get_coef can be used to extract the the name of exposure and mediator from identified significant mediation pathways with related coefficient estimates. ```{r} get_coef(res_SMAHP = res) ```