Package: FracFixR Type: Package Title: Compositional Statistical Framework for RNA Fractionation Analysis Version: 1.1.0 Date: 2026-05-11 Authors@R: c( person("Alice", "Cleynen", email = "alice.cleynen@cnrs.fr", role = c("aut", "cre"), comment = c(ORCID = "0000-0001-8083-0204")), person("Agin", "Ravindran", role = "aut"), person("Nikolay", "Shirokikh", email = "nikolay.shirokikh@uwa.edu.au", role = "aut", comment = c(ORCID = "0000-0001-8249-358X"))) Description: A compositional statistical framework for absolute proportion estimation between fractions in RNA sequencing data. 'FracFixR' addresses the fundamental challenge in fractionated RNA-seq experiments where library preparation and sequencing depth obscure the original proportions of RNA fractions. It reconstructs original fraction proportions using non-negative linear regression, estimates the "lost" unrecoverable fraction, corrects individual transcript frequencies, and performs differential proportion testing between conditions. Supports any RNA fractionation protocol including polysome profiling, sub-cellular localization, and RNA-protein complex isolation. License: CC BY 4.0 Encoding: UTF-8 LazyData: true Depends: R (>= 4.0.0) Imports: future.apply (>= 1.8.1), nnls (>= 1.4), ggplot2 (>= 3.3.5), dplyr (>= 1.0.7), RColorBrewer (>= 1.1-2), tidyr (>= 1.1.3), matrixStats (>= 0.60.0), aod (>= 1.3.1), parallelly (>= 1.30.0), stats, utils, future, grDevices Suggests: testthat (>= 3.0.0), knitr, rmarkdown RoxygenNote: 7.3.2 VignetteBuilder: knitr NeedsCompilation: no Packaged: 2026-07-10 06:37:31 UTC; root Author: Alice Cleynen [aut, cre] (ORCID: ), Agin Ravindran [aut], Nikolay Shirokikh [aut] (ORCID: ) Maintainer: Alice Cleynen Repository: https://cran.r-universe.dev Date/Publication: 2026-05-11 08:39:24 UTC RemoteUrl: https://github.com/cran/FracFixR RemoteRef: HEAD RemoteSha: d77c96c6362c3c75d0a9c0d67580f42ac21e14c7